Quentin Dupieux was born in 1974. At the relatively young age of 12, he found a camera and started taking pictures of anything and everything. At 17, he began to play music to illustrate his images, and bought his first synthesiser. In 1997, Laurent Garnier of F Com bought a car from Mr. Oizo's father. Garnier then found out about Dupieux's talents and let him direct the music video for his song "Flashback". Later Dupieux was signed by F Com Records where he released his first EP fittingly titled, "#1".

Flat Beat is a track with a repeated bass loop. As stated in an interview with XLR8R magazine he said that it took him 2 hours on a lazy Sunday afternoon with a Korg MS-20 to make. It is commonly argued that the loop was originally produced by DJ Dee Kline and features on his collaborative track with Pixie entitled 'Darren'. Dupieux is however most known for this track, which became a hit throughout Europe in 1999 and was featured in a series of Levi's jeans TV commercials. Dupieux also directed the ad, which featured a yellow puppet named Flat Eric nodding his head to the sound of the track while riding in an old beat-up car. After "Flat Beat", Oizo spent two months locked up in a studio, creating his first full album, Analog Worms Attack. The Flat Beat EP has sold over 3 million copies.

Analog Worms Attack is Oizo's first album, released in 1999. The album's namesake is derived from the album's production – it was composed entirely using analog equipment. The record scratching effects were provided by Mr. Oizo's good friend, DJ Feadz. "Flat Beat" appears as a bonus track, after the 5 minutes of silence following the final track "Analog Wormz Sequel". There are a total of 3 singles off of this album. A version of "No Day Massacre" without any record scratching exists on the Flat Beat EP, titled "Monday Massacre". The record is generally slow, with noticeable hip-hop influences in the style of Beastie Boys.

Moustache (Half a Scissor) was Mr. Oizo's second studio album, released in 2005 after a long hiatus. This album was composed using exclusively computers, as he had decided to eschew the use of analog equipment. The extensive time it took to remodel his studio and master the use of computers in composing electronic music is cited as the reason for the long hiatus between Analog Worms Attack and Moustache (Half a Scissor). Before its public release, a promo CD was put on the internet featuring 3 tracks not on the original release: "CPU","Nazis", and "A Nun". Some songs on the promo are different then the released album versions, for example "Nurse Bob" is much longer on the release while the promo version splices vocal samples into a much shorter version. This album is his only to not be released on vinyl. There is another hidden track in this album, about 1 minute and 15 seconds after the last track "Vagiclean", there is a short, untitled song full of bass and strange noises.

Lambs Anger is the first studio album released by Ed Banger Records, the label Mr. Oizo chose after his split with F Communications, who notoriously referred to Moustache (Half a Scissor) as "unlistenable". It is composed of numerous dance tracks in a more mainstream electro style that are far less experimental-sounding than those featured on Moustache. "Positif" and "Pourriture" were chosen as the album's singles.

In his early years, Mr. Oizo used a Korg MS-20, an Akai S1000, and an Atari 1040 computer. Mr. Oizo is known currently for strong use of computers in his music. As he stated in an interview with XLR8R magazine, he started using computers to avoid having to plug in 4 different appliances, and because the result of music is more or less the same. His first album to switch to all computers was Moustache (Half a Scissor). In Lambs Anger, Mr. Oizo used a Macintosh G5 running Logic Pro to compose all of the songs. During his DJ sets, he uses a Macbook for the music alongside two CDJs for mixing.

He directed the French natural thriller flick Rubber, which has an United States Video on Demand release.